SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix : Stock solution

Sample ID

Vail #

: 13

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin033.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 1:09:22 AM : 1/23/2007 2:34:21 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin033.lcd

mV

1Det.A Ch1

3

4

5

2000

6

1500

1

2

1000

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 9584608 8719358 21482780 18068450 13766317 12166339 83787852

Height

Area %

Name

1 2 3 4 5 6 Total

15.517 19.702 20.352 21.373 22.229 25.522

1492343 1219371 2360406 2311083 2092979 1968443 11444625

11.439 10.406 25.639 21.565 16.430 14.520 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin033.lcd