SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 750 mg / 100 ml :Trl 2

Vail #

: 31

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP002.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 2:19:13 AM : 1/23/2007 3:09:21 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP002.lcd

mV

1Det.A Ch1

200

3

150

1

100

4

5

2

50

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.529 19.684 20.362 21.373 22.234 25.530

478028 401702 1091994 545128 382010 122902 3021764

85286 57571 153766 89813 68586 21932 476953

15.819 13.294 36.138 18.040 12.642 4.067 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP002.lcd