SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2000 mg / 100 ml : Trl 2

Vail #

: 50

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II008.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 7:07:50 PM : 1/23/2007 3:05:39 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II008.lcd

mV

1Det.A Ch1

500

3

400

300

1

4

5

200

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1320439 1090118 3006238 1512807 1101225 336094 8366920

Height

Area %

Name

1 2 3 4 5 6 Total

15.462 19.630 20.308 21.330 22.192 25.499

230805 158305 421639 248164 192750 60035 1311697

15.782 13.029 35.930 18.081 13.162 4.017 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II008.lcd