SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 750 mg / 100 ml :Trl 1

Vail #

: 30

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP001.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 1:33:29 AM : 1/23/2007 3:08:13 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP001.lcd

mV

1Det.A Ch1

200

3

150

1

100

4

5

2

50

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.517 19.676 20.354 21.366 22.228 25.522

465764 391249 1068054 533845 376121 119712 2954744

82453 56273 150253 87827 67629 21438 465874

15.763 13.241 36.147 18.067 12.729 4.052 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP001.lcd