SPDS Set 1 ERP Book

CHON-003

Question

Response

General

AOAC Method Number:

CHON-003

Chondroitin by IR and Dimethylene Blue (DMBB) spectrophotometry

Method Title:

Method Author(s):

Not stated

Reviewer Name:

Aniko Solyom

Colorimetric method for the quantification of chondroitin sulfate. According to the method description, the method is applicable for the quantification of chondroitin sulfate in raw materials and finished products, such as tablets.

Summary of Method

Method Scope / Applicability:

General Comments About the Method: Method Clarity

Detailed SOP style

Relatively quick and cheap method for the analysis of raw materials and probably simple finished products. 1. Chondroitin-DMMB complex will aggregate immediately, therefore the reading time should be the same for each sample/standard. Room for significant human error! 2. Not a specific method for complex samples. If anything (including adulterants)in the sample reacts with DMMB and absorbs at 525 nm, the results are not valid.

Pros/Strengths

Cons/Weaknesses

Supporting Data

General Comments Method Optimization

Not Provided Performance Characteristics

Calibration curve: 5-15 microgram chondroitin sulfate (only 3 point calibration curve) Chondroitin sulfate content of the

Analytical Range: