SPDS Set 1 ERP Book

ANTH-001

Vaccinium fruit anthocyanins are more complex than Acai anthocyanin profiles. There are 3 common species that share the common name Acai. The different Acai species each have a slightly different anthocyanin profile (refer to Yamaguchi et al. (2015) Food Chemistry, 179:137-151, Schauss et al. 2006 JAFC 54:8598-8603, Schauss et al. 2006 JAFC 54:8598-8603). Authors did not identify three Acai species available (Euterpe edulis, E. precatoria*, or E. oleracea*; *are the typical commercially available species). As diode array detectors (DAD) are more commonly available to researchers and commercial laboratories, authors should also have included one as well. DAD peak spectra provide valuable anthocyanin identification information. Just separating the 2 predominate Acai anthocyanins that the authors’ report (possibly incorrectly identified), while ignoring the additional anthocyanins in Acai is not sufficient. Examining the minor anthocyanins in a fruit sample can sometimes determine whether a sample is adulterated or not (see 3 references mentioned previously). The anthocyanin identifications and the Acai species used in this work are questionable. Supporting Data Anthocyanin SMPR requested an anthocyanin fingerprinting method for Vaccinium fruit. This method demonstrated cyanidin-glucoside and cyanidin-rutinoside could be separated and quantified. But, a method that can only detect two anthocyanins is not sufficient. Will have to demonstrate Vaccinium fruit anthocyanins can be separated (probably will need a longer analytical column to achieve this.) Need to include DAD. There is long-term confusion if only plant common names are used; genus and species information have to be included. If possible, analyze NIST cranberry sample, though even this needs to be done with caution. While NIST published anthocyanin data of this cranberry reference material, unfortunately their cranberry anthocyanin identifications were incorrect (see “Lowenthal et al. (2013). Analytical and Bioanalytical Chemistry, 405, 4451-4465.”; this paper identified cranberry anthocyanins incorrectly, or NIST reference material was not authentic cranberry). Performance Characteristics

General Comments

Method Optimization

Analytical Range:

Not applicable. SMPR was for a qualitative method.

LOQ:

Not applicable. SMPR was for a qualitative method.