SPDS Set 1 ERP Book

ANTH-001

Question

Response

General

AOAC Method Number:

ANTH-001

Determination of Anthocyanins in Brazilian and Floridian Açaí (Euterpe oleraceae Mart) Using LC-MS/MS

Method Title:

Method Author(s):

FDA (Kevin Tran, Ralph H. Vocque and Robert E. Smith)

Reviewer Name:

Melissa Phillips

Method describes a semi-quantitative LC-MS/MS method for determination of Cy3Glu and Cy3Rut in Acai fruit products for authentication. Scope is only to acai products (which are not included in SMPR scope except as part of the exclusivity panel) and/or only based on Cy3Glu and Cy3Rut. Applicability to other products (or exclusion of non-Vaccinium) is not demonstrated or likely. Great as a quick method for detecting adulteration in acai products; not a great fit for the SMPR does not meet the needs of SMPR. Chromatography likely cannot be extended to include the necessary Vaccinium anthocyanins without significant extra work. Applicability not demonstrated to the authenticity and exclusivity panels. Supporting Data Needs significantly more work before consideration by ERP for first action. clear as stated simple and fast; seems to work well for acai

Summary of Method

Method Scope / Applicability:

General Comments About the Method:

Method Clarity

Pros/Strengths

Cons/Weaknesses

General Comments

Method Optimization

some MS parameters optimized Performance Characteristics

0.0 - 0.8 mcg/mL, assuming reference standard purity of 100%

Analytical Range: