SPDS Set 1 ERP Book

ANTH-002

analysis or different processing methods used to calculate both standards and samples together at different wavelengths to ensure consistency. Clarity required around standard concentration calculation. The standard listed in method is salt form. No discussion of handling/drying of standard material covered. Results are then Cyanidin-3-chloride, or converted to Cyanidin-3-glucoside with correction factor of 1.38 as listed in 8.6.4. This factor is incorrect, it should be 1.3926, molecular mass data from Chem Spider:(Cyanidin-3-glucoside 449.384 Da; Cyanidin chloride 322.697 Da) No QC, acceptance criteria or performance criteria in the method. Either a NIST or other validated control needs to be included. No expiration dates on solutions, standards or samples, or storage conditions of such. The filter type used to filter samples is not stated. There is no discussion of light sensitivity of the analytes and working under yellow lights or using actinic glassware. There is no information in regard to the ruggedness of the method when dealing with dietary supplement matrices. Comparing "fingerprints" of analytes may be difficult between different HPLC systems/conditions as well as growing conditions/harvest times. This could be elucidated with example chromatograms. Supporting Data No example chromatograms are included, so cannot comment on resolution of peaks of interest or the sensitivity. Sonication of samples and/or other extraction solvents might be explored for increased extraction efficiency of analytes. Performance Characteristics

General Comments

Method Optimization

Analytical Range:

18 to 180 mg/L standard curve range.