SPDS Set 1 ERP Book

ANTH-003

Question

Answer

General

AOAC Method Number:

ANTH-003

Anthocyanin Profiles by HPLC with DAD and MS Detections

Method Title:

Method Author(s):

Bob Durst

Reviewer Name:

John Szpylka

Different fruits listed as "authentic" were squeezed into juices. The juices were analyzed using a Synergi Hydro- RP 80A column using different mobile phases to allow detection by DAD or by MS. Thirteen fruits covering seven species were studied (V. darrowii not covered). 25 peaks were identified covering 5 classes of anthocyanidins (does not include pelargonidin as listed in the SMPR). Sugar additions to the oxonium were galactoside, glucoside, and arabinoside; and acetic acid acylated on the sugar. 1. The method shows the potential to profile anthocyanins in a variety of fruits. The method targets known compounds using two detection technologies. 2. While the ease of sample preparation and the quality of the chromatographic analyses is appealing, the SMPR- listed scopes of the method were not fully covered in this report. One matrix type was studied and no exclusivity panel fruits were included. 3. The potential of the method is evident and should be considered if the method is more deeply evalauted using the SMPR requirements. A general description on the preparation of samples was present. Details on sample handling, preparation, and storage were not listed. This could be due to the simplicity of collecting juice from the fruits It must be noted the only form of material tested was the juice collecting from squeezing. No dry product, dry extracts, capsules, softgels, or tablets were studied.

Summary of Method

Method Scope / Applicability:

General Comments About the Method:

Method Clarity