SPDS Set 1 ERP Book

PDE5-002

Question

Answer

General

AOAC Method Number:

PDE5-002

Screening Method for Phosphodiesterase Type 5 (PDE5) Inhibitors in Dietary Ingredients and Supplements

Method Title:

Method Author(s):

Eike Reich, Thi Kieu Tiên Do

Reviewer Name:

Katerina Mastovska

Samples are extracted with methanol. Extracts are analyzed by high performance thin layer chromatography (HPTLC) with UV and MS detection, for detection of adulteration with sildenafil, vardenafil, tadalafil, hydroxyacetildenafil, homosildenafil, thiohomosildenafil, acetildenafil, acetaminotadalafil, propoxyphenyl hydroxyhomosildenafil, hydroxyhomosildenafil, and hydroxythiohomosildenafil. Screening of sildenafil, vardenafil, tadalafil, hydroxyacetildenafil, homosildenafil, thiohomosildenafil, acetildenafil, acetaminotadalafil, propoxyphenyl hydroxyhomosildenafil, hydroxyhomosildenafil, and hydroxythiohomosildenafil. Tadalafil and its analogs have limited solubility in methanol, which is used as the extraction/dilution solvent in the method Overall clear description, except for the MS detection. Which MS spectrometer was used? How were the eleven target compounds identified/confirmed using MS? Relatively simple method for screening of PDE5 inhibitors but there is not enough information to evaluate the method performance. Potentially limited scope - reference standards are necessary for detection in HPTLC. Estimated detection limit seems to be 20-fold higher than the AOAC 2014.012 SMPR low concentration of 100

Summary of Method

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Method Clarity

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