SPDS Set 1 ERP Book

PDE5-004

Question

Response

General

AOAC Method Number:

PDE5-004

Testing for Phosphodiesterase Type 5 (PDE5) Inhibitors in Dietary Supplements

Method Title:

Method Author(s):

Not stated

Reviewer Name:

Teresa Cain

This is bioluminescent assay method to screen for presence of PDE5 inhibition in a test sample based on a commercially available assay kit which is sensitive and reliable. Scope of method is to screen for PDE5 inhibiting compounds as adulterants in dietary supplements. This method shows value in being a quick and easy screening method to detect the presence of drug adulterants in dietary supplements. Method uses a commercially available Assay Kit with clear instructions. *Method is simple and easily applied as a rapid screening method for the presence of compounds with PDE5 inhibition. *Sensitivity of the method allows dilution of possible interfering matrix components. *False positives are extremely unlikely due to dilution of the sample matrix. *Method states that assay may be adaptable to a hand- held device. Non-specific method without capability of identifying analyte present Supporting Data

Summary of Method

Method Scope / Applicability:

General Comments About the Method:

Method Clarity

Pros/Strengths

Cons/Weaknesses

General Comments

NA

Method Optimization

NA