SPDS eBook March 2017

3/10/2017

Existing Methods (General) ▪ Abundance of published methods, mainly using HPLC-UV or HPLC-MS ▪ UV/Vis spectrophotometry (Folin-Ciocalteu) used for total phenolic compounds ▪ HPTLC, CE-UV infrequently used ▪ Established methods include: ▪ Official methods of the United States Pharmacopeia and European Pharmacopoeia ▪ American Herbal Pharmacopoeia ▪ HPLC-UV for phenolic compounds in Echinacea angustifolia root ▪ HPLC-UV for phenolic compounds in Echinacea pallida root ▪ HPLC-UV for phenolic compounds in Echinacea purpurea root and herb ▪ SLV for phenolic compounds in Echinacea angustifolia , Echinacea pallida , and Echinacea purpurea root and herb by Brown et al. (2011)

Official Methods ▪ United States Pharmacopeia ▪ Echinacea angustifolia root, powdered root, and powdered extract: HPLC-UV for phenolic compounds ▪ Echinacea pallida root, powdered root, and powdered extract: HPLC-UV for phenolic compounds ▪ Echinacea purpurea root, powdered root, and powdered extract: HPLC-UV for phenolic compounds ▪ European Pharmacopoeia ▪ Echinacea angustifolia root (whole or cut): HPLC-UV for phenolic compounds ▪ Echinacea pallida root (whole or cut): HPLC-UV for phenolic compounds ▪ Echinacea purpurea root (whole or cut): HPLC-UV for phenolic compounds ▪ Echinacea purpurea dried herb (whole or cut): HPLC-UV for phenolic compounds

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