SPDS eBook March 2017

Existing Methods (General)

HPLC Method for Potency and Purity Test

HPLC :

Regular System

▪ ▪ ▪ ▪ ▪ ▪

, 5 P , 4.6x 250mm

Column :

XTerra RP 8

UV Detection: Mobile Phases

257 nm

PO 4

buffer

A: 25 mM NaH 2

B: ACN

Features

Easy and Simple to do; Short; Low Cost; Reliable

Existing Methods (General)

A Typical Chromatogram of SAMe Tablets

mAU

SAMe

250 200 150 100 50

0

0

min

2

4

6

8

10

12