SPDS eBook March 2017

Existing Methods (General)

HPLC Method for Potency and Purity Test Some Factors about Racemization to Consider:

• Synthetic: S/R = 50/50 • Natural: 2-4 Months to S/R = 50/50 • S and R are Convertible

Existing Methods (General) A Sample Chromatogram of SAMe Degradation Products

mAU

Adenosine

400

350 300 250 200 150 100 50 0

DMTA

SAM

SAH

PTSA

4

8

10

12

min

6

0

2