SPDS eBook March 2017

Background

Definition: • The major polysaccharide in aloe is glucomannan  which is consisted of mannose (major) and glucose  (minor) with 1,4‐β‐linked backbone;   • The mannose moieties are highly acetylated and are  referred to acetylated glucomannan polysaccharides;

2/3/6-OAc

2/3/6-OAc

6

HO

HO

HOH 2 C O

HOH 2 C

O

HOH 2 C

O

O

1

3 4 5

O

O

O

Structure of the major aloe polysaccharides

HO

2

HO

HO

OH

Glc p

Man p

y

x

Man p

z

Background

Summary of current methods used in Aloe  qualification (identification) and quantitation analysis: • 1 H NMR – Qualification of aloe raw material and product; – Quantitation of polysaccharides by analysis of the content  of acetyl groups; – Quantitation of organic acids including acetic acid, lactic  acid,  malic acid and isocitric acid;