SPDS eBook March 2017

Background

Summary of current methods used in Aloe  qualification (identification) and quantitation analysis  ( cont’d ): • Example of aloe identification by  1 H NMR

Aloe whole leaf

Unique fingerprint only found in aloe acetylated polysaccharides

Acetyl groups

Isocitric acid lactone Isocitric acid

Isocitric acid

Isocitric acid lactone

Aloe inner gel

Background

Summary of current methods used in Aloe  qualification (identification) and quantitation analysis  ( cont’d ): • HPLC – qualification of aloe organic acid fingerprint, including  malic, lactic, citric, fumaric acid, isocitric, and isocitric acid  lactone.  Isocitric and its lactone are whole leaf markers; • Example of aloe HPLC fingerprint for identification; 

Whole leaf Inner gel Rind