SPDS eBook March 2017

Background

Summary of current methods used in Aloe  quantitation analysis: • 1 H NMR – polysaccharides, monosaccharides, organic  acids;  • HPLC – organic acids; • HPAEC‐PAC – organic acids, disaccharides,  monosaccharide, oligosaccharides; • GC – organic acids, monosaccharides including existed  monosaccharides or hydrolyzed from polysaccharides; • Colorimetric – quantitation of aloe polysaccharides by  photometric analysis;  

Background

Summary of current methods used in Aloe  quantitation analysis ( cont’d ): • GPC‐RI (Reflective Index) – Provide fingerprint of aloe polysaccharides and their  molecular weight and size; – Require polysaccharide standards, such as dextran,  pullulan;  • GPC‐RI‐MALLS (Multi Angle Light Scattering)  – Measure absolute molecular weight; – Don’t require polysaccharide standards for quantitation;