SPDS eBook March 2017

Background

Summary of current methods used in Aloe quantitation  analysis ( cont’d ): • 1 H NMR vs. GPC‐RI‐MALLS – NMR quantitation only works on the acetylated polysaccharides; – Degrees of acetylation on the aloe polysaccharides are varied  depending on manufacturing process; – GPC‐RI‐MALLS quantitation covers all the polymers eluted from GPC  including acetylated or non‐acetylated polysaccharides or other  polymers such as proteins;

SMPR of Aloe Identification Key Points

• Identification of acetylated glucomannan  polysaccharides derived from Aloe Vera in dietary  ingredients and dietary supplements;  • Candidate methods should be able to differentiate  acetylated glucomannan polysaccharides derived  from whole leaf and/or inner leaf products from gel;  • Any analytical technique that meets the method  performance requirements is acceptable;   • May require developing aloe polysaccharide  standards for qualification;