SPDS eBook March 2017

3/10/2017

Background

Free alpha amino acids and related compounds

β‐alanine

Alanine

Arginine Cystine Histidine

Asparagine

Aspartic Acid Cysteine Glutamine Glycine Isoleucine Leucine Phenylalanine Proline

Glutamic Acid Hydroxyproline

Lysine Serine

Methionine

Taurine

Threonine Tryptophan Tyrosine

Valine

SMPR Key Points

Method Performance Requirements

Acceptable Criteria

Parameters

0.04 ‐ 100

Analytical Range (%)

LOQ  (%)

≤0.04

Recommended  LOD (%)

≤0.01

For individual free amino acid components measured.

Ranges (%)

0.04 ‐10

> 10

Recovery (%)

98 – 102

90 ‐ 107

≤ 3

% RSD r

≤ 5

≤4

% RSD R

≤ 8

For individual free amino acid components measured.

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