SPIFAN Nutrients ERP Book_9-29-15

BVit-02 - MEF FORM FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 1

Accuracy - Spike Recovery

Spike Recovery

Product

SPIFAN Code

Analyte

Nr. replicates (n)

Native level ug/100g as RTF

Low (~50% Fort.)

High (~200% Fort.)

Avg (%)

RSD % Avg (%)

RSD (%)

Adult Nutritional RTF High Fat, placebo Adult Nutritional RTF High Protein, placebo

00820RF00 00821RF00 00847RF00

Total B1 Total B1 Total B1 Total B1 Total B1 Total B1 Total B1 Total B1 Total B2 Total B2 Total B2 Total B2 Total B2 Total B2 Total B2 Total B2 Total B3 Total B3 Total B3 Total B3 Total B3 Total B3 Total B3 Total B3 Total B6 Total B6 Total B6 Total B6 Total B6 Total B6 Total B6 Total B6

3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps 3 days x 2 reps

0.3 0.4 1.0 0.1

94.9 97.7 99.1 93.7

4.9 4.3 5.7 6.4 1.8 1.7 0.7 4.3 3.7 1.6 2.3 2.7 1.8 1.8 2.0 2.9 1.8 2.1 7.8 5.9 6.3 6.8 1.8 2.1 1.6 5.8 4.8 3.2 2.4 1.8 2.1 1.3 2.5 1.5 2.8 2.2

93.4 95.4 97.0 92.4

5.8 5.5 7.0 7.8 1.8 1.2 1.5 6.1 4.6 1.7 2.7 2.8 1.0 2.4 1.9 2.6 3.2 2.3 6.8 3.8 5.3 4.9 2.4 2.4 3.3 7.0 4.5 2.7 2.7 1.9 3.4 2.4 2.6 2.5 3.8 2.8

Child Formula Powder, placebo Infant Elemantal Powder, placebo

00796RF

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

410057652Z 410457651Z

73.5 74.6 98.6

100.2 101.1

100.8 102.5

Infant Formula Powder Soy Based Infant Formula RTF Milk Based, placebo

E10NWZC

98.7 96.9 97.8 97.2 96.5 98.0 95.1 99.9

94.5 95.6 96.4 95.6 98.4 98.9 96.7 99.7 99.6 95.4 97.9 97.8

EV4H2Q

3.3

MEAN

Adult Nutritional RTF High Fat, placebo Adult Nutritional RTF High Protein, placebo

00820RF00 00821RF00 00847RF00

48.7 41.2 26.7

Child Formula Powder, placebo Infant Elemantal Powder, placebo

00796RF

6.2

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

410057652Z 410457651Z

159.0 101.0 125.0

100.2

Infant Formula Powder Soy Based Infant Formula RTF Milk Based, placebo

E10NWZC

97.3

EV4H2Q

53.3

100.7

MEAN

98.1

Adult Nutritional RTF High Fat, placebo Adult Nutritional RTF High Protein, placebo

00820RF00 00821RF00 00847RF00

34.2 24.4 10.4

102.1 101.7 105.4 104.5 103.0 102.3 101.8 105.3 103.2 100.2 96.8 99.7 98.0 99.1 99.3 98.6 99.0 100.1

103.1 102.9 105.7 103.8 110.7 105.6 100.3 103.4 104.4

Child Formula Powder, placebo Infant Elemantal Powder, placebo

00796RF

5.7

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

410057652Z 410457651Z

775.0 787.0 843.0

Infant Formula Powder Soy Based Infant Formula RTF Milk Based, placebo

E10NWZC

EV4H2Q

23.2

MEAN

Adult Nutritional RTF High Fat, placebo Adult Nutritional RTF High Protein, placebo

00820RF00 00821RF00 00847RF00

4.6 6.3 6.0 1.0

99.5 98.9 99.1 98.4

Child Formula Powder, placebo Infant Elemantal Powder, placebo

00796RF

Infant Formula Powder Partially Hydrolyzed Milk Based Infant Formula Powder Partially Hydrolyzed Soy Based

410057652Z 410457651Z

54.4 56.1 58.8

100.1 100.5

Infant Formula Powder Soy Based Infant Formula RTF Milk Based, placebo

E10NWZC

97.6 99.6 99.2

EV4H2Q

3.3

MEAN

Additional information to be completed by SD

Was a portion of the product spiked? Were all spike recovery samples taken from the same spiked product? Were multiple portions of the product spiked and each spiked product analyzed? Were products spiked before they were weighed into extraction vessels? Were all spike recovery samples prepared on multiple days? Were spike recoveries calculated from theoretical additions? Or was spiking solution analyzed and the results used to calculate spike recoveries? Were all spike recovery samples prepared on the same day? What form of the nutrient was spiked into the product? What kind of solution or solvent was used to deliver the spike to the product? Were products weighed into extraction vessels before spiking?

yes no yes yes

no no

yes yes no

water 3% low-13% high Mixed std

What percentage of the spiked sample was the spiked was the spike blanc? (How much was product diluted by spike?)

SPIFAN ERP Checklist v1.0