SPIFAN Nutrients ERP Book_9-29-15

Carot-01 FOR ERP USE ONLY DO NOT DISTRIBUTE

Table 1. Precision and Spike recovery results for beta-carotene Beta-carotene Performance Analytical range 10 µg/kg – 2.8 mg/kg Limit of quantitation (LOQ) ≤ 10 µg/kg Linearity (10 points) 0.01 – 10 mg/L: r 2 = 0.9993 Repeatability (n = 6-fold, RSD r ) 9.2 µg/kg (SM) 4.5 % 110 µg/kg (SM + spike) 0.9 % 520 µg/kg (IF) 4.2 % 2820 µg/kg (IF + spike) 2.9 % Reproducibility (n = 6 duplicates, RSD R ) 9.2 µg/kg (SM) 110 µg/kg (SM + spike) 520 µg/kg (IF) 2820 µg/kg (IF + spike) Recovery (n = 6) 110 µg/kg (SM + spike) 97 % 2820 µg/kg (IF + spike) 109 %

SM = skimmed milk (liquid). IF = infant formula (powder). Results are based on the total of cis and trans isomers.

Table 2. Precision and Spike recovery results for lutein Lutein

Performance

Analytical range

71 µg/kg – 4.6 mg/kg

Limit of quantitation (LOQ)

≤ 71 µg/kg

0.02 – 3.3 mg/L: r 2 = 0.9999

Linearity (10 points) Repeatability (n = 6-fold, RSD r )

not detectable (SM) 65 µg/kg (SM + spike)

-

2.4 % 2.1 % 2.4 %

1210 µg/kg (IF)

5020 µg/kg (IF + spike) not detectable (SM) 65 µg/kg (SM + spike) 1210 µg/kg (IF) 5020 µg/kg (IF + spike) 65 µg/kg (SM + spike) 5020 µg/kg (IF + spike)

Reproducibility (n = 6 duplicates, RSD R )

Recovery (n = 6)

98 %

101 %

SM = skimmed milk (liquid). IF = infant formula (powder). Results are based on the total of cis and trans isomers.