SPIFAN Nutrients ERP Book_9-29-15

FOS-04 FOR ERP USE ONLY DO NOT DISTRIBUTE

Fructose Concentration (  g/mL) = Fructose wt. (g) x 1,000,000  g/g x Purity 500 mL

Where: Purity = %Purity (from CoA)/100%

2. Working standards – Concentrations (in  g/mL) are as follows:

WS4 = Fructose stock * 50 /100

WS3 = Fructose stock * 25/100

WS2 = Fructose stock * 5/100

WS1 = Fructose stock * 5/ 200

3. Sample fructan (g/100g) – Obtain the peak areas for fructose and glucoheptose for each level of working standard. Fit the peak area ratios (responses) and corresponding standard concentrations, in  g/mL, to a quadratic model (not forced through the origin). Interpolate the sample concentration in  g/mL using the corresponding fructose response parameters. Calculate interpolated sample concentration using the proper factors, as defined in 3.a -3.c.:

Total Fructan (g/100g) = Interpolated fructose (in µg/mL) x DF (dilution factor) x CF(commodity factor) SWF(sample weight factor)

a. Sample Weight Factors (SWF)- Calculated as follows:

RTF :

SWF = SW 1

(g) x SW 2

(g) x 10,000

SWF = SW 1

(g) x SW 2

(g) x 10,000

Commodity:

SWF =

PW (g) x SW 1

(g) x SW 2

(g) x 10,000

Powder:

{ PW (g) + WW (g)}

SW 1 = weight of material delivered to a volumetric flask (RTF, or reconstituted powder) and diluted as per sample table guidelines. For example, a RTF product of 0.21-1.0% fructan would have a SW 1 of 7 g (±10%) into a 200 mL volumetric flask.

SW 2 = weight of solution from volumetric flask per sample table guidelines (volumetric dilution of SW 1

). Value should be

0.2 g ± 10%.

PW = weight of powder product weighed to be reconstituted (5 g ± 10%).

WW = weight of water used to reconstitute powder products (40 g ± 10%).

Dilution factor (DF)– Calculated as follows:

b.

Low-level fructan (RTF 0.03-0.2%, powder 0.27-1.0%)

DF = 50 mL

x

Commodity Factor x

2 mL

Mid-level fructan (RTF 0.21-1.0%, powder 1.1-10%)

DF = 200 mL

x

Commodity Factor x

2 mL

High-level fructan (RTF 1.1-5.0%, powder 11-45%)

DF = 500 mL

x

Commodity Factor x

2 mL

Commodity DF (46-100%)

DF = 1000 mL

x

Commodity Factor x

2 mL

c. Commodity factors (CF) – As in Table 2-4 (Type determined by qualitative ID methodology in Part 1 if not known).

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