SPIFAN Nutrients ERP Book_9-29-15

GOS-02 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

12.4.

GOS is calculated as follows (g GOS/ 100 g reconstituted product):

Calculatio

GOS/ (g GOS ofn

product) g 100

10.4) (paragraph (µg/mL) sample hydrolyzed - non from galactose B 10.3) (paragraph (µg/mL) sample hydrolyzed from galactose A : samples the of tion Quantifica =

= =

from lactose C

10.4) (paragraph (µg/mL) sample hydrolyzed - non

10.5) (paragraph (µg/mL) blank enzyme from galactose D

=

:n calculatio feed Ready to

   

   

) 342 180 *(C-D) -B- (A b

(

)

c

25 1000 * 50*

d

100 *

a

1000

Amount

GOS

product g 100 per

*

k

=

(mg) feed Ready to

Reconstitu

powders ted

liquid and

concentrat

: es

   

   

) 342 180 *(C-D) -B- (A b

(

)

c

25 1000 * 50*

d

100 *

a

1000

product

(g) water (g) +

Amount

GOS

product g 100 per

*

*

k

=

(g) product

Reconstitu

(mg) product ted

product g 100 n to calculatio d collection fraction e preparativ by dilution c (mL) flask c volumetri volume b mg/mL n to calculatio a : legend = = =

= =

calculate factor to k

1.485 galactose; from GOS

used is

The k-factor (degree of polymerization) is determined as:

n 180 162 180 where n is the average number of galactose moieties in GOS molecules. n k ⋅ ⋅ + =

For Vivinal GOS, which is the predominant product on the market, n was determined as 1.71, which results in a k-factor of 1.485 (Coulier, 2009). We used this k-factor throughout the SLV. It was not possible to determine the k-factor in IFT samples, for which the GOS source is unknown.

13.

References:

De Slegte, J. (2002) Determination of trans-Galactooligosaccharides in Selected Food Products by Ion-Exchange Chromatography : Collaborative Study. J AOAC Int 85(2) , 407-423.

Coulier, L. (2009) In-Depth Characterization of Prebiotic Galactooligosaccharides by a Combination of Analytical Techniques. J Agric Food Chem 57 , 8488-8495.

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