SPIFAN Nutrients ERP Book_9-29-15

GOS-02 SLV FOR ERP USE ONLY DO NOT DISTRIBUTE

Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

Method:

6.

6.1.

GOS fractionation •

Carbopac PA100 column plus guard

Eluent A = 1 M NaOH Eluent B = 1 M NaAc

• • • • • • • •

Eluent D = Milli-Q Flow 1 mL/min

Column temperature 20°C Tray temperature 10°C Pressure upper limit = 4000 psi

Sample injection 25 µL

Table 5.3: Elution scheme for fractionation Time (min) Eluent A (%) Eluent B (%) Eluent C (%) Eluent D (%) Curve 0.00 10 4 0 86 5 1.10 10 4 0 86 5 4.00 50 40 0 10 5 8.00 50 40 0 10 5 8.10 10 4 0 86 5 21.0 10 4 0 86 5

6.2.

GOS quantification •

Carbopac PA1 column plus guard

Eluent A = 300 mM NaOH

• • • • • • • •

Eluent B = 1 M NaAc Eluent D = Milli-Q

Flow 1 mL/min

Column temperature 20°C Tray temperature 10°C Pressure upper limit = 3000 psi

Sample injection 25 µL

Table 5.4: Elution scheme for quantification Time (min) Eluent A (%) Eluent B (%) Eluent C (%) Eluent D (%) Curve 0 5 0 0 95 5 20 5 0 0 95 5 33 32 0 0 68 5 35 32 0 0 68 5 39 32 50 0 18 5 43 32 50 0 18 5 45 32 0 0 68 5 48 5 0 0 95 5 63 5 0 0 95 5

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