SPIFAN SP Meeting September 17, 2016

AOAC Official methods

– AOAC 961.16 (Titrimetry) – AOAC 944.08 (Ash-Distillation-Titration) These 2 methods are not suitable for determining low levels of fluoride in infant formula and adult nutritional products. – Microdiffusion and F-ISE ( Microdiffusion and fluoride-specific electrode determination of fluoride in infant foods: collaborative study - Dabeka, R. W., McKenzie, A. D. J. AOAC., 64, (4): 1021-1026,1981) Results from collaborative study were not accepted by AOAC. Method not published.

September 2016

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German Official Method (Fluo-01) Amtliche Sammlung von Untersuchungsverfahren, nach Paragraph 35 LMBG.(L 49.00- 7): 2pp., 2000 - Analysis of foods. Determination of fluoride in dietetic foods with an ion-sensitive electrode. • Only validated on 2 dietetic products • Modified and validated with 30 Healthcare products (Nestlé method): – PLOQ RTF: ≈50 μg/100g (Dilution factor 2 with TISAB) – Good RSDr (< 5%) / RSDiR (< 8%) / Recovery (83%-109%)* *Bad Recovery for products with high protein/thickeners/caseinates due to non specific binding of measured free fluorides Conclusion: method not sensitive & not applicable to products with high protein / thickener/caseinates content + SLV (SPIFAN kit) September 2016 8