SPIFAN Stakeholder Panel (September 23, 2017)

Analyte Definition-III

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Rationale:

1. 2-/3-MCPD in free form or being present as fatty acid esters and fatty acid esters of glycidol are food processing contaminants [1-3]. 2. Free MCPD isomers might occur in various foods due to heat treatment, by acidic hydrolysis of food ingredients, or by migration from food contact materials. [3] 3. Bound MCPD/glycidol origins in major parts from the application of elevated temperatures during oil refining. Consequently all infant and pediatric formula and related nutritionals containing refined edible oils and fats are prone to also contain 2- & 3-MCPD esters and glycidyl esters. 4. The bound analytes are expected to occur in infant formulae at levels being predominant in relation to free MCPD contents.

[1] R. Weisshaar; Eur. J. Lipid. Sci. Technol ., Editorial, 110, 2008 , 671-672 [2] R. Weisshaar, R. Perz; Eur. J. Lipid. Sci. Technol . 112, 2010 , 158-165 [3] C. Crews et al.; Food Addit. Contam . 19 (1), 2002 , 22-27

Analyte Definition-IVa

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Implications:

1. As during sample preparation 3-MCPD might be converted into glycidol and vice versa , candidate methods should include measures to avoid or control these transformations. 2. Artefact formation of 3-MCPD and glycidol might take place during sample preparation. Candidate methods should have shown to be not significantly impacted by these effects. 3. In case that samples do contain analytes with medium, short-length or poly-unsaturated fatty acids, candidate methods based on direct determination of the single esters likely underestimate analyte contents due to the limited availability of reference compounds.