SPSFAM ERP

Single Lab Validation of Megazyme’s Fructan Assay Kit (K-FRUC) Method for the Determination of Fructan (Inulin, FOS, Levan and Branched Fructan) in Animal Food (Animal Feed, Pet Food, and Ingredients)

Objective

Evaluation of the Megazyme Fructan Assay Kit method (K-FRUC; updated with inclusion of endo -levanase) for the measurement of total dietary fructan, such as inulin, levan, branched fructans including agave fructans) and fructo-oligosaccharides (FOS), in animal feed, pet food, and the corresponding ingredients. The method can distinguish dietary fructans from interfering compounds such as free glucose, fructose, sucrose, starch and other polysaccharides.

Authors

Barry V. McCleary, Lucie M. J. Charmier, Vincent A. McKie, Ciara McLoughlin and Artur Rogowski.

Status

Unpublished but commercially available.

Conclusion The method developed meets the standard method performance requirements that can be tested in a single lab. This method is believed to be, currently, the only method that can specifically measure inulin, levan and Agave (branched) fructan in foods, feeds and pasture grasses.

Date of Issue 29 th June, 2018.

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