SPSFAM ERP

The results of the SLV are summarized in Table 1 .

Table 1. Results of the SLV for the Fructan Assay Kit.

AOAC SMPR ® 2018.002

Megazyme (K-FRUC)

0.21 to 98.4 a

Operating range (% w/w)

0.2 to 100

Limit of quantitation (LOQ) (% w/w)

0.119 b

0.20

RSD r RSD r RSD r RSD R RSD R Fructan) Fructan)

, % (0.2 to 1% w/w Fructan) , % (>1 to 10% w/w Fructan)

7 5 3

4.74 3.59 2.96 8.47 6.36

, % (>10 to 100% w/w

, % (0.2 to 1% w/w Fructan)

14 10

, % (>1 to 10% w/w

RSD R

, % (>10 to 100% w/w

6

5.77

Fructan)

a Precise range dictated by fructan content in samples tested. b Based on replicate measurements for a sample with ~ 1% (w/w) fructan.

2. Introduction Fructans are defined as any compound where one or more fructosyl-fructose linkage constitutes a majority of the linkages. This refers to polymeric material as well as oligomers as small as the trisaccharide, kestose. Material included in this definition may or may not contain D-glucosyl substituents. Fructans are widely distributed in the plant kingdom. They are present in monocotyledons, dicotyledons and in green algae. Fructans differ in molecular structure and in molecular weight. They may be classified into three main types: the inulin group, the levan group and the branched (agave-type) group. The inulin group consists of material that has mostly or exclusively the (2→ 1) fructosyl-fructose linkage. Levan is material that contains mostly or exclusively the (2→ 6) fructosyl-fructose linkage. The branched group has both (2→ 1) and (2→ 6) fructosyl-fructose linkages in significant amounts (e.g. graminan from Gramineae and agave fructan).

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