SPSFAM ERP

3. Definitions a) Animal and pet foods. —Material consumed or intended to be consumed by animals other than humans that contributes nutrition, taste, or aroma or has a technical effect on the consumed material. This includes raw materials, ingredients, and finished product. (AAFCO) b) Fructans. —Any DP ≥ 3 carbohydrate with one or more fructosyl-fructose linear or branched linkages which constitute a majority of the linkages in which fructose is the major constituent and glucose content is 33% or less. Examples include inulin, levan, branched fructans (e.g., agave fructans), and fructooligosaccharides (FOS). 4. General Information This method is based on the Fructan Assay Kit (Megazyme cat. no. K-FRUC) which is available for purchase from Megazyme directly at www.megazyme.com Technical support can be provided to the user via email (contact scientist@megazyme.com ). 5.1 Scope of the Method i. Target analyte – Fructan, including inulin, FOS (non-reducing), levan and branched fructans (e.g. agave fructan). ii. Matrices – For this validation, the Fructan Assay Kit (K-FRUC) was tested with the following matrices: 1. Animal feed and pet food samples Brett Brothers swine feed (purchased from Glanbia, Ireland). Purina GoCat dry cat food (purchased from Tesco Ireland, Greystones, Ireland). Top Spec TopChop alfalfa (purchased from TopSpec Equine Ltd, Middle Park Farm, Pickhill, Thirsk, North Yorkshire, YO7 4JN, UK). 5. Method c) Ingredients. —The base materials used in the formulation of animal and pet foods.

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