SPSFAM ERP

Timothy grass hay (Lot no. 171201)(supplied by Alison Muirhead, Grass Seed Direct, Menstrie Mains, Menstrie, Clackmannanshire, Scotland). Rye grass hay (supplied by Dr Joe Panozzo, Seed Phenomics and Quality Traits Agriculture Victoria Research, Horsham, Victoria, Australia). 2. Barley grain/flour Conventional barley flour (Lot no. 60301a) (from Megazyme, Bray, Ireland). Barley MAX ® (from Paul Sims, The Healthy Grain Pty. Ltd., South Yarra, Victoria, Australia). 3. Pure, or high purity commercial of purified fructans: Raftiline ® (Native Chicory Inulin – supplied by BENEO Tienen , Aandorenstraat 1, 3300 Tienen, Belgium). Frutafit ® agave fructan (supplied by Sensus, Borchwerf 3, Roosendaal, The Netherlands). Levan (cat. no. P-LEVAN; Lot: 171114 purified from Timothy grass - from Megazyme, Bray, Ireland).

ii. Certified reference materials (CRMs) Certified reference materials are not currently available for fructan.

iii. Expression of results – The concentration of fructan is expressed as % w/w of the sample analyzed.

5.2 Method Principle Products to be analyzed are suspended in water in tubes that are heated in a boiling water bath to dissolve the fructan. Sucrose in the sample is hydrolyzed at 30 o C by a specific sucrase enzyme (1), which has no action on lower degree of polymerization (DP) FOS, such as 1-kestose and 1,1-kestotetraose.

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