SPSFAM ERP

( c ) Disposable 2.0 mL polypropylene microfuge tubes .- e.g. Sarstedt cat. no. 72.691 (www.sarstedt.com).

(d ) Glass test tubes .- 16 120 mm, round bottom, 17 mL.

(e) Pyrex screw cap culture tubes (25 x 150 mm).- with PTFE lined phenolic caps (e.g. Fisher cat. no. 14-933D).

( f ) Volumetric flasks .-50 and 100 mL.

(g) Micro-pipettors.- e.g. Gilson Pipetman® (100 µ L and 200 µ L).

(h) Positive displacement pipettor .- e.g. Eppendorf Multipette® with 5.0 mL Combitip® (to dispense 0.2 mL aliquots of sucrase mixture and 0.1 mL aliquots of fructanase, and other solutions and buffers); with 50 mL Combitip ® (to dispense 5.0 mL aliquots of PAHBAH Working Reagent). (i) Bottle-top dispenser.- e.g. Brand ® S Digital 2.5 - 25 mL cat. no. 4600351 to dispense 25 mL of 200 mM sodium acetate buffer (pH 5.0).

(j) Water bath .-Maintaining 30± 0.1°C and 40 ± 0.1°C.

(k) Boiling water bath .- Deep-fat fryer filled with water at 95-100°C.

(l) Analytical balance.

(k) Spectrophotometer .- Operating at 410 nm.

( e ) Vortex mixer .

( f ) pH Meter .

( g ) Stop-clock timer .

5.4 Chemicals and Reagents

All reagents should be of analytical purity grade.

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