SPSFAM ERP

The LOQ of 0.119 % (w/w) meets the LOQ requirement of the AOAC SMPR ® 2018.002.

6.2.6 Trueness (Bias)

Accuracy of the Fructan Assay Kit (K-FRUC) method was assessed by comparison of the mean fructan content (x) obtained for a suitable reference material with a specific reference value ( ). There are no official certified reference materials for fructan however the reference material used for this analysis was Raftiline (Native Chicory Inulin) which is a commercially available, almost pure, inulin.

Relative Bias, b (%) is calculated as: % = − × Table 6. Relative Bias Reference value (Fructan; % w/w)

100

Mean (Fructan; % w/w) Relative Bias; b (%)

97.15 -2.85

n

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The accuracy of the Fructan Assay Kit (K-FRUC) method is extremely high with a calculated Relative Bias of -2.85% for a pure inulin sample.

6.2.7 Precision The precision of the Fructan Assay Kit (K-FRUC) method was assessed using 10 milled samples. For each sample, duplicate extractions were processed and applied to the Fructan Assay Kit (K-FRUC) method on 4 separate occasions by 2 independent analysts. The fructan content of the samples tested covered a working range of 0.2 to 98.4% (w/w) fructan. The repeatability (%RSD r ) and reproducibility (%RSD R ) across this sample set was extremely high.

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