SPSFAM ERP

Repeatability (%RSD r ) was less than or equal to 2.96% for samples containing 10 to 100% (w/w) fructan, less than or equal to 3.59% for samples containing 1 to 10% (w/w) fructan and less than or equal to 4.74% for samples containing 0.2 to 1% (w/w) ) was less than or equal to 5.77% for samples containing 10 to 100% (w/w) fructan, less than or equal to 6.36% for samples containing 1 to 10% (w/w) fructan and less than or equal to 8.47% for samples containing 0.2 to 1% (w/w) fructan precision ( Table 8 ). The levels of repeatability and reproducibility obtained with the Fructan Assay Kit method indicate a very high level of precision and meet the requirements of the AOAC SMPR ® 2018.002 for all of the specified ranges of fructan. fructan precision ( Table 7 ). Reproducibility (%RSD R

Table 7. Repeatability. For each sample, duplicate extractions were processed and applied to the assay on 4 separate occasions by a single analyst.

Fructan, % (w/w), mean ±2 SD, (%CV)

Interday: Fructan, % (w/w), mean, ±2 SD, (%RSD r )

Sample

n1

n2

n3

n4

99.8 ± 4.1

98.2 ± 1.2

98.3 ± 0

97.2 ± 1.5

98.4 ±2.6

Raftiline Inulin (native chicory fructan)

2.04

0.61

0.00

0.77

1.34

97.5 ± 1.6

98.3 ± 2

99.2 ± 1

98.6 ± 0.9

98.4 ±1.7

P-­‐LEVAN #171114

0.83

1.01

0.50

0.45

0.88

85.7 ± 3.5

86.4 ± 2

86.8 ± 3

83.2 ± 1.5

85.6 ±3.6

Frutafit Agave fructan

2.07

1.17

1.75

0.91

2.10

14.4 ± 0.8

14.3 ± 0.1

14.8 ± 0.4

14.8 ± 0.5

14.6 ±0.6

Timothy grass #171201

2.93

0.50

1.50

1.61

2.21

14.4 ± 0.2

14 ± 1.4

14.7 ± 0.7

14.3 ± 0.6

14.4 ±0.8

Barley Max

0.60

4.92

2.46

2.27

2.95

11 ± 0.4

10.8 ± 0.3

10.4 ± 0.6

10.4 ± 0.1

10.7 ±0.6

Rye Grass

1.73

1.25

2.98

0.46

2.96

2.01 ± 0.03 1.96 ± 0.01 1.98 ± 0.12 1.94 ± 0.1

1.97 ±0.09

Brett Brother swine feed

0.78

0.31

3.05

2.64

2.16

1.7 ± 0

1.7 ± 0.1

1.7 ± 0.1

1.5 ± 0.1

1.6 ±0.1

Barley Flour #60301a

0.32

2.80

3.04

2.78

3.59

0.98 ± 0.03 0.99 ± 0.18

1.03 ± 0

0.97 ± 0.02

0.99 ±0.09

Purina GOCat dry cat food

1.73

9.18

0.16

0.78

4.29

0.2 ± 0.02

0.2 ± 0.02 0.21 ± 0.01 0.2 ± 0.03

0.2 ±0.02

Top spec alfalfa

4.20

3.82

2.91

8.53

4.74

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