SPSFAM ERP

Sugar Profile by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection: A Multi-Laboratory Validation (MLV) Study Tom Vennard, Andrew Ruosch, David Ellingson Covance Food Solutions, 3301 Kinsman Boulevard, Madison, WI 53704, USA Introduction Numerous different analytical methods for sugar analysis are currently employed to measure nutritionally relevant mono- and di-saccharides in a wide range of raw material and finished product food types to fulfil Nutrition Labeling and Education Act (NLEA) requirements. Current compendial sugar test methods either have limited scope to specific matrices or utilize older analytical technology that are impacted by interferences from complex matrices. To address this problem, AOAC Int. issued a call for methods for determination of Sugars in Animal Feed, Pet Food, and Human Food based on the AOAC Standard Method Performance Requirements (SMPR) document 2018.001 approved by the AOAC Int. Stakeholder Panel on Strategic Food Analytical Methods (SPSFAM) [1] . The method performance requirements are summarized in Table 1 .

Table 1. Method performance requirements in the AOAC SMPR 2018.001 [1]

Fructose, Glucose, Sucrose, Maltose, Lactose, and Galactose

Analytes

Analytical range, %*

0.1-5

>5-50

>50-100

Recovery, %

90% –110%

95% – 105%

97% – 103%

% RSD r % RSD R

≤ 7%

≤ 5% ≤ 8%

≤ 3%

≤ 10% ≤ 4% * Reported as the individual sugars (fructose, glucose, sucrose, maltose, lactose, and galactose)

This report describes a multi-laboratory validation (MLV) study of a high performance anion- exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method for quantification of fructose, glucose, sucrose, maltose, lactose, galactose, and isomaltulose in a broad range of food ingredients and finished products. The MLV study was conducted with testing at 4 different Covance laboratories located in Battle Creek, MI, USA; Harrogate, UK; Madison, WI, USA; and Singapore, labeled as BC, HAR, MSN, and SGP in this report. The evaluated matrices cover all sectors of the AOAC food pyramid from Appendix F: Guidelines for Standard Method Performance Requirements [2] – see Table 2 and Figure 1 , which summarizes the matrices included in this validation and the corresponding AOAC food pyramid sector. Table 2 also