SPSFAM ERP

(u) (v) (w) (x) (y)

Plastic filter funnels. Plastic cups with lids.

Ultrasonic bath.

Disposable syringes.

Syringe filters, 0.45 μm GHP or equivalent.

(z) High pressure mixing tee (IDEX Health & Science p/n U-466) or equivalent. (aa) Resistance tubing: 5 ft. of 0.007 in. ID PEEK, yellow (Thermo Fisher p/n 37007) or equivalent. (ab) Analytical column.— Thermo CarboPac PA20, 150 × 3 mm, Part No. 060142. (ac) Guard column.— Thermo CarboPac PA20 guard, 30 × 3 mm, Part No. 060144. (ad) Borate trap column. — Thermo BorateTrap, 50 × 4 mm, Part No. 047078. (ae) Anion trap column. — Thermo ATC 500, 24 × 4 mm, Part No. 075976.

B.

Materials and Reagents Water (H 2 O).— Ultra pure.

(a) (b) (c) (d) (e) (f)

Ethanol (EtOH ).—190 proof, HPLC grade.

Acid washed celite.

Sodium hydroxide (NaOH) .—50% ( w / w ). Sodium hydroxide (NaOH).— 1N. Sodium azide (NaN 3 ).— Reagent grade.

(g) Carrez I solution.— 3.6% ( w/v ), (Fisher part number SC910116), or equivalent. (h) Carrez II solution.— 7.2% ( w/v ), (Fisher part number SC910216), or equivalent.

C.

Reference Standards D-(+)-Glucose , ≥99% D-(+)-Galactose , ≥99% D-(-)-Fructose , ≥99%

(a) (b) (c) (d) (e) (f) (g) (h)

Sucrose , ≥99% Lactose , ≥99%

D-(+)-Maltose monohydrate , ≥99%

Palatinose hydrate , ≥99% D-(-)-Arabinose , ≥99%

D.

Preparation of Reagent Solutions

(a) Extraction solution (50% ethanol in water).—Combine equal volumes of ethanol and water in an appropriate container. Mix thoroughly. (b) Mobile Phase A (Water).—Measure 2000 mL of water into a 2 L graduated cylinder. Vacuum degas or helium sparge for approximately 5 minutes. Place in appropriate mobile phase container and keep under inert gas while in use on the instrument.