SPSFAM ERP

0.42 0.43 0.44 0.50

-2.0 +0.6 -0.1 -0.1

Table 4. IC gradient program for routine analysis Time (min) Flow (mL/min) %A

%B

%C

%D

0.00

0.5 0.5 0.5 0.5 0.5 0.5

90 90

10 10

0 0

0 0 0 0 0 0

13.00 25.00 27.00 27.01 30.00

0 0

0 0

100 100

90 90

10 10

0 0

Table 5. IC gradient program for additional clean-up following injection of high fiber samples Time (min) Flow (mL/min) %A %B %C %D 0.00 0.5 90 10 0 0 5.00 0.5 90 10 0 0 10.00 0.5 0 0 100 0 27.00 0.5 0 0 100 0 27.01 0.5 90 10 0 0 30.00 0.5 90 10 0 0

Table 6. IC gradient program for post run system standby Time (min) Flow (mL/min) %A %B

%C

%D 100 100

0.00

0.5 0.5 0.5

0 0

0 0

0 0 0 0

20.00 20.01 21.00

90 90

10 10

0 0

0.15

Table 7. IC gradient program for post run system shutdown Time (min) Flow (mL/min) %A %B

%C

%D 100 100

0.00

0.5 0.5 0.5

0 0

0 0

0 0 0 0

20.00 20.01 21.00

90 90

10 10

0 0

0.01