SPSFAM ERP

E. Specificity The SMPR lists several possible interferences. This table of potential interferants is shown in Figure 3 .

Figure 3. Potential interferences from AOAC 2018.001 SMPR [1]

Each of these possible interferences has been assessed with the method and the successful demonstration of this method to address each interference category is discussed below. The non-target mono- and di-saccharides, sugar alcohols, and amino sugars interference evaluation was completed by injecting solutions of the pure reference solutions of a variety of compounds in these categories and comparing the retention times to the elution of the target sugar analytes. The chromatographic overlay of this evaluation is shown in Figure 4 . Galactose, glucose, sucrose, fructose, isomaltulose, and maltose are all sufficiently resolved from these potential interferences to allow accurate determination. Figure 4. Chromatographic overlay of peaks of interest relative to potential interferences from mono- and di-saccahrides, sugar alcohol, and amino sugar. Retention times for identifying competing peaks: fucose = 3.5 min, maltitol = 5 min, rhamnose = 6.3 min, glucosamine = 7.9 min, xylose = 11.8 min, mannose = 11.9 min, ribose = 15 min, isomaltose = 20.2 min, lactulose = 21.1 min, raffinose = 22.0 min, stachyose = 23.7 min