SPSFAM ERP

Previously published work [ 3] by this group demonstrated the efficacy of the extraction solvent system (50:50 ethanol:water) and molecular weight cut off filtration to effectively eliminate enzymatic and bacterial degradation that can cause changes to the individual analyte concentrations in certain matrices (pet food, animal feed). In addition to the inclusion of maltitol (sugar alcohol) in the chromatographic specificity assessment the analytical column documentation demonstrates that sugar alcohols elute well before any peaks of interest in this method. [4] To address the possible interference from organic acids, higher degrees of polymerization of glucose, and hydroxylated compounds (such as artificial sweeteners) single solutions of representative compounds in these categories were injected and the interference with the analytes of interest was assessed. The chromatographic overlay of these potential interferants is shown in Figure 5 . Citric acid, Sorbic acid, Lactic acid, Benzoic acid, Aspartame, and Sucrolose, representing the organic acids and hydroxylated compounds, showed no peaks in the chromatogram and therefore no chromatographic interferences. The maltodextrin, which represents the high degree of polymerization glucose category, showed small peaks for maltose and glucose. This result is expected as small amounts of these analytes of interest are known to be present in maltodextrin.