SPSFAM ERP

Figure 5. Chromatographic overlay of peaks of interest relative to potential interferences from organic acids, glucose polymer, and hydroxylated compounds.

Due to the separation and detection modes salts, such as sodium chloride, do not interfere with the measurement of the analytes of interest with this method. Previous validation by this group on a different IC column using HPAEC-PAD (CarboPac PA1) showed no impact to the instrument response to 1:1 dilution of a calibration standard with either water or 10% sodium chloride (yielding a final concentration of 5% NaCl). Each solution was injected in triplicate. The data are shown in Table 15 . Based on the similarity of the separation between the CarboPac PA1 and CarbPac PA20 methods and the identical detection modes of the assays, sodium chloride has been demonstrated to not interfere with the measurement of the sugar compounds using this method.

Table 15. Specificity for high sodium chloride concentrations, triplicate injections of each sample

Average Glucose Area

Average Fructose Area

Average Sucrose Area

Average Lactose Area

Average Maltose Area

Sample

W5 with water

30.2 30.4 0.4%

38.1 37.9 0.6%

29.4 29.8 1.6%

28.7 28.3 1.5%

30.4 31.0 2.0%

W5 with 5% NaCl

Relative % difference

To address interferences from hydrolytic activity (acidic activity), starches (glucose polymers), and amine containing compounds (pet and animal food, protein powders, meat sources), a broad range of matrices were analyzed in the validation that contain these potential interfering compounds. The validation on theses matrices demonstrated acceptable method performance to address the specificity related to these classes of possible interferences. The method demonstrates acceptable performance relative to all potential interferants outlined in the SMPR.

F.

Stability of Reference and Sample Solutions