SPSFAM ERP

e. Dispense as needed into auto sampler vials, do not pre-vial and store on the counter 4 . f. Stable for six months stored at room temperature with a parafilm seal. 5. 5 mg/mL Sodium Chloride Standard Solution a. Weigh 0.5 g sodium chloride and dispense into a 100 mL volumetric flask. b. Add 40 mL of deionized water using a 20 mL class A glass pipette (2 x 20 mL) and swirl to dissolve the salt. c. Add 60 mL of acetonitrile using a 20 mL class A glass pipette (3 x 20 mL). d. Stopper, invert and shake to mix well. Do not dilute to volume. e. Dispense as needed into autosampler vials, do not pre-vial and store on the counter. f. Stable for six months stored at room temperature with a parafilm seal. 6. 60 % Acetonitrile:40 % Water Diluent Solution a. Dispense 150 mL HPLC reagent grade acetonitrile into a 250 mL volumetric flasks using a 50 mL class A glass pipette (3 x 50 mL). b. Dispense 100 mL of deionized water using a class A glass pipette. c. Mix contents well. d. Stable for six months stored at room temperature 6. Diluant - 5 mg/mL of ribose (Internal Standard) in water

a. Weigh 5 g grams of ribose and dispense into a 1 L volumetric flask. b. Add 500 mL of deionized water and dissolve by hand mixing. c. Dilute to volume with deionized water. d. Stable for 2 weeks stored at room temperature.

Sample Preparation: 1.

Grind samples as homogeneous as possible in air-tight, dark containers to a fine powder. Upon receipt of the delivered samples to the storage area, the handling and storage of the samples should be in accordance with good laboratory practices. Allow refrigerated or frozen samples to equilibrate to room temperature before weighing. Sample Size: The method was optimized to achieve a LOQ of 0.1% for the labeling sugars in various matrices with consideration to moisture and sample weight as denoted in the yellow region as a guideline.

3 Accelerate temperature acclimation to RT by ~30 seconds immersion in hot water from a sink spigot. 4 Over time, acetonitrile will evaporate out of vials crimp sealed.