SPSFAM EXPERT REVIEW PANEL

AOAC SPSFAM ERP REVIEW: MAIN FORM

Submission Date

2017-08-31 08:54:01

Name

Xu-Liang Cao

E-mail

xu-liang.cao@hc-sc.gc.ca

Organization

Health Canada

Title of Method

Bisphenol A in water

AOAC Candidate Method Number (e.g. ALN-01)

BPA-01

Applicable SMPR

BPA

Summary:

This HPLC with electrochemical detection method was submitted by Nico Reinhound from Antec Scientific. It is based on an ALEXYS system in combination with a solid phase sample pre-concentration step. A 1 mL loop is filled with sample, which was carried onto the pre-column by a solvent. The concentrated sample from the pre- column was flushed onto the analytical column by another solvent for separation, and the analyte was detected by an electrochemical cell with an electrode at optimum potential of 900 mV. Detection limit is 0.5 nmol/L, or 114 ng/L (0.114 ug/L). The submitted method only demonstrated its application to the analysis of BPA in drinking water, while its applicability to the other beverage products specified in SMPR is unknown due to the missing information on method performance parameters. It is not clear how the detection limit of the submitted method was estimated, and it is unlikely it was estimated according to the procedures specified in the SMPR. It is also not clear whether the claimed detection limit (0.5 nmol/L as stated in the summary or 0.3 nmol/L stated in the text) is LOD or LOQ. The claimed detection limit of 0.3 nmol/L (or 0.0684 ug/L) meets the requirements for both LOD and LOQ specified in SMPR. Repeatability meets the SMPR, but it was demonstrated using the standard solutions instead of real samples.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

The detection limit of the submitted method may have not been estimated according to the definition specified in the SMPR.

No. The author could refer to some available documents on laboratory safety, such as the AOAC official method of analysis.