SPSFAM EXPERT REVIEW PANEL

1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference. 2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. method performs within the SMPR Method Performance REquirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. 1. Based on the supporting information, were there any additional steps in the evaluation of the method that indicated the need for any additional precautionary statements in the method? 2. Does the method contain system suitability tests or controls as specified by the SMPR? If not, please indicate if there is a need for such tests or controls and which ones. 3. Is there information demonstrating that the

Yes

No commercial reference materials are identified in the SMPR, and no reference material (commercial or in-house prepared) was used in the method.

For the 2 matrices and 3 concentrations tested, yes.

cola at 5 ug/L; 90.8% recovery, 1.0% RSDr formula at 1 ug/L; 88.4% recovery, 1.5% RSDr formula at 2 ug/L; 85.7% recovery, 2.7% RSDr

formula at 1 ug/L; 84.4% recovery, 7.4% RSDR formula at 2 ug/L; 85.8% recovery, 5.3% RSDR

No LOD, LOQ, or analytical range is given. LOQ is reported as 0.5 ug/L based on a similar method reported in the literature using canned energy drinks.

Additional matrices should be tested, and higher concentrations should be tested. I have concerns about the loading capacity of the SPE cartridges, and would like to see explicit instructions about sample dilution if necessary.

No information provided about calibration at all. This should be included as well.

No

Some blank samples were included. No check standards were tested.