September 27, 2015 SPSFAM Meeting - Meeting Book

Existing methods LC/MS/MS method to target allergen peptides

Sample Preparation

Identification

Peptide - Q1 m/z

Peptide - MS/MS m/z

Allergen protein

Peptides

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*

Digest

Extract Analysis

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*

0

200 400 600 800 m/z

Screening

LC Ionization MS/MS (MRM mode)

Existing LC/MS/MS Methods • Revision of the sample Extract proteins from food sample (1 hour in

buffer)

preparation utilizing  automated online SPE • Total sample preparation  time down to 4.5 hours • Results achieved through  modification: • Prep‐time reduced by 4x • Sensitivity improved by 2‐4x • LC‐MS/MS run time  reduced by nearly 2x • Addition of more allergens  (milk, egg, peanut, treenuts,  gluten, sesame, mustard)

Centrifuge and collect extract (30 min)

Reduce, alkylate, and digest with trypsin ( 2.5 hours )

Filter digested extract (15 min)

Perform automated online SPE clean-up ( 0 hours hands-on time )

LC-MS/MS analysis