September 27, 2015 SPSFAM Meeting - Meeting Book

Regulatory Guidance / Issues • Food Allergen Labeling and Consumer Protection Act of  2004. – Labeling requires the word “Contains” followed by the name of the  major food allergen – for example, “Contains milk, wheat” – or a  parenthetical statement in the list of ingredients – for example,  “albumin (egg)” • Regulation (EU) No 1169/2011 – on the provision of food information to consumers entered into  application on 13 December 2014 . The obligation to provide  nutrition information will apply from 13 December 2016 .

Fitness for Purpose

XICof+MRM(71pairs):Exp1 929986/1116601DaID:[e]ELINSWVESQTNGIIRfromSample1(E100mrmepi4allergen1612081)ofE100mrme , . . ...

Max 71000cps . . .

• Benefits of LC‐MS/MS:

2.5e4 2.8e4

Egg peptides

2.0e4

– Improved throughput  through multiplexing – Multiple allergens in  one injection – Multiple fragments for  each allergen detected – More confidence for  fewer false positive or  false negative results

1.5e4

1.0e4

12.69

Intensity,cps

5000.0

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0 11.0 12.0 13.0 14.0 15.0 16.0

Time,min

XIC of+MRM (71 pairs):Exp 1, 688.830/833.400 DaID: [p]DLAFPGSGEQVEK fromSample 1 (P100mrmepi4 allergen 161208 1) ofP100mrmepi4 ...

Max. 9900.0 cps.

0.0 5.0e4 1.0e5 1.5e5 2.0e5 2.5e5 3.0e5 Intensity, cps 0.0 1.0e5 2.0e5 3.0e5 4.0e5 5.0e5 6.0e5 7.0e5 8.0e5 8.8e5 Intensity,cps

Peanut peptides

9.67

7.27

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0 11.0 12.0 13.0 14.0 15.0 16.0

Time min , XIC of+MRM (71 pairs):Exp 1, 684.350/1183.570 DaID: [m]ALNEINQFYQK fromSample 1 (M100mrmepi4 allergen 161208 1) ofM100mrmepi4 all...

Max. 7.7e4 cps.

Milk peptides

10.08

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0 11.0 12.0 13.0 14.0 15.0 16.0

Time,min

Multi-allergen analysis