Stakeholder Panel Meeting Book - September 6, 2014

 The thiachrome reaction was developed in 1935  for the analysis of thiamine in biological matricies  Thiachrome at pHs above 8.0 has a strong blue  fluorescence (ex 375nm, em 432–435nm)  Thiachrome analysis has been used for most of  the analytical data available for the B 1 content in  food.  Thiachrome is used as the determinative step in  the official methods of the USP, AOAC, and Food  Chemical Codex as well as many others 

The Dietary Reference Intake (DRI), or daily requirement, for  Vitamin B 1 (Thiamine) is as follows:

children 0‐5 months children 6‐11 months children 1‐3 years children 4‐8 years children 9‐13 years males 14 and older

0.2 mg/day  0.3 mg/day  0.5 mg/day  0.6 mg/day  0.9 mg/day  1 2 mg/day .  1.0 mg/day 

females 14‐18

females 19 and older 1.1 mg/day  During pregnancy and lactation 1.4 mg/day.

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