Stakeholder Panel Meeting Book - September 6, 2014

B 6

Regulatory Information Min Max US Infant Formula  0.035 mg/100kcal 0.245 mg/L* ‐‐ EU Infant formula 0.035 mg/100kcal 0.245 mg/L* 0.10 mg/100kcal 0.70 mg/L  EU FSMP 0.08 mg/100 kcal 0.56 mg/L* 0.24 mg/100kcal 1.6 mg/L Codex Infant Formula 0.035 mg/100 kcal 0.175 mg/100kcal 0.245 mg/L* 1.225 mg/L Aus/NZ Infant  Formula 0.038 mg/100kcal 0.266 mg/L* 0.15 mg/100kcal 1.05 mg/L China (GB) Infant  Formula 0.035 mg/100 kcal      0.245 mg/L* 0.175 mg/100kcal 1.225 g/L

*based on 70 kcal/100mL

Current Official Testing Methods B 1  AOAC 942.23 (Food) and 986.27 (IF)  HPLC with  fluorescence detection, acid hydrolysis, enzyme  treatment, and pre‐column thiachrome  formation.  GB 5413.11, EN 14122 and BIS 5398 all employ a  very similar approach as the AOAC methods with  the EN method allowing for post‐column  thiachrome conversion, and the BIS method also  including the microbiological method option.

23

Made with