Stakeholder Panel Meeting Book - September 6, 2014

Current Official Testing Methods B 6  AOAC 985.32 microbiological, acid hydrolysis, and  enzymatic treatment.  AOAC 2004.07 & EN 14164 HPLC with fluorescence  detection, acid hydrolysis, enzymatic treatment combined  with chemical transformation of all three forms of B6 to  Pyridoxine.   EN 14663 HPLC with fluorescence detection, acid  hydrolysis, enzymatic treatment all three forms of B 6 quantitated separately

What do we need to test?

Vitamin

Forms

What should we What should we  test? report?

B 1

Thiamin (free) TMP

Thiamine Chloride  Hydrochloride

Total B 1

TPP TTP

B 2

Riboflavin (free) FMN FAD

Riboflavin

Total B 2

B 3

Nicotinic Acid Nicotinamide

Total B

Nicotinamide

3

B 6

Pyridoxine Pyridoxal

Pyridoxine

Total B 6

Pyridoxamine PNP,PLP,PMP

25