Total Collaborative Study Protocol_Solus One Salmonella v1 1

reader or a Dynex DS2 instrument plate reader), where a result of an OD 450

< 0.200 is considered to be 

≥ 0.200 is considered to be positive for the target pathogen. 

negative for the target pathogen and OD 450

General Information 

Salmonella  is typically a motile, non‐spore forming, Gram‐negative, rod‐shaped bacterium in the 

family Enterobacteriaceae ; although non‐motile variants, such as  Salmonella Gallinarum and  Salmonella

Pullorum, also exist. The genus  Salmonella  is divided into two species that can cause illness in humans, 

Salmonella bongori and  Salmonella enterica , the latter being characterized as being the greatest public 

health concern (4).  

Salmonella  species and subspecies are also subdivided into serotypes, based on the Kaufmann‐

White typing scheme first published in 1934, which differentiates  Salmonella  strains by their surface and 

flagellar antigenic properties.  Salmonella  species are commonly referred to by their serotype names. For 

example,  Salmonella enterica  subsp. enterica  is further divided into numerous serotypes, including 

Salmonella Enteritidis and  Salmonella Typhimurium, which are common in the U.S. (4).  

Every year, Salmonella  is estimated to cause approximately one million foodborne illnesses in the 

United States, which correlates to approximately 64% of pathogen bacterial foodborne outbreaks. Most 

people infected with  Salmonella develop diarrhea, fever, and abdominal cramps that last 12 to 72 hours 

after infection. The illness usually lasts 4 to 7 days, and most people recover without treatment. 

However, for some people the diarrhea may be severe enough to require hospitalization. 

Any raw food of animal origin, such as meat, poultry, milk and dairy products, eggs, seafood, and 

some fruits and vegetables may harbor  Salmonella . The bacteria can survive and cause illness if the raw 

products are not cooked to a minimum internal temperature.  Salmonella  can also cause foodborne

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