Total Collaborative Study Protocol_Solus One Salmonella v1 1

Polypropylene Tubes .— 2 mL and 15 mL and 25 mL. 

( i )

( j ) Frit filters .— Optional for matrices with a lot of particulate , CAT No. FRI001. 

( k ) 70% v:v Ethanol .— For preparation of the Solus One  Salmonella Supplement. 

( l )

Deionized water .

( m )  Sterile collection sponge for sampling environmental surfaces .— World Bioproducts EZ Reach 

Sponge Sampler with Hi Cap Neutralizing Buffer, or equivalent.

( n )  Sterile collection swab for sample environmental surfaces . ( o )  Letheen Broth neutralizing buffer .

Apparatus 

( a )  Refrigerator .— capable of maintaining 2–8°C. 

( b )  Incubators .— capable of maintaining 37 ± 1°C and 41.5 ± 1°C. 

( c )  Circulating water bath .— capable of maintaining 42–50°C. 

( d )  Heating block or water bath .— capable of maintaining 85–100°C. 

( e )  Autoclave for decontamination of samples .  ( f )  Homogenizer  (g)       Measuring cylinder .— capable of measuring 2 L. 

( h )  Vessel or container .— capable of storing 2 L. 

( i ) 

Filter laboratory blender bags . 

( j )  Sterile collection sponge and swabs for sampling environmental surfaces .  ( k )  Serological Pipette Bulbs (Automatic Pipette) .— For sampling and delivering of 1–10 mL.

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