Total Collaborative Study Protocol_Solus One Salmonella v1 1

( c )  Start heating block or heat water bath to 85–100°C prior to initiating testing.  ( d ) Allow heated samples to cool to room temperature (15–25°C). This may be accelerated by  placing the test tubes in cold water for 5 min.  ( e ) Where appropriate frit filters will be used with denatured matrices that contain high  concentrations of particulates thereby minimizing sample handling errors.  ( f )  Change pipette tips in between samples. 

Pathogen Detection (Automated Sample Preparation Method) 

( a ) Equilibrate test kit to room temperature (15–25°C) an hour before use.  ( b ) Start the Dynex DS2 and allow instrument to conduct the ‘Self‐Test’.  ( c )  Load required materials: pipette tips, reagents, washing buffer, etc.  ( d ) Empty waste.  ( e ) Start heating block or heat water to 85–100°C prior to initiating testing.  ( f )  Allow heated samples to cool to room temperature (15–25°C). This may be accelerated by 

placing the test tubes in cold water for 5 min. 

( g )  Where appropriate use frit filters with denatured matrices that contain high concentrations of 

particulates to minimize sample handling errors. 

Sample Enrichment and Preparation  

( a )  Raw Beef Trim (375 g test portions)

Pre‐warm 1,125 mL of Solus BPW (ISO) Medium supplemented with Solus  Salmonella  supplement

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